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Thermo Scientific Nalgene Rapid-Flow Filters Brochure |
the last line of defense
against contamination
Thermo Scientific
Nalgene
™
Rapid-Flow
™
Filters
All Nalgene filters now have the Rapid-Flow multi-column
membrane-support system. This proprietary system
provides a uniform, consistent separation between
touchpoints with the membrane, minimizing gap stress
to maintain optimal flow.
Other filters use a radial spoke support system.
The gaps between spokes lack uniformity and
consistency in membrane support, leading to
increased stress and distortion. The result?
Suboptimal flow rate and throughput.
From the outside, bottle top filters look alike. But, when you look beneath the membrane,
glaring differences become apparent. Differences that are critical to the way the filter performs
and how it impacts your work.
Thermo Fisher Scientific introduces filter units with the unique Thermo Scientific Nalgene
Rapid-Flow design. These filters feature a support system engineered to allow membranes
to work at maximum efficiency — providing superior performance and the last line of defense
against contamination.
Membrane
Consistently consistent.
Mind the gaps.
Membrane
The Rapid-Flow Advantage.
Performance on many levels.
NALGENE BOTTLES MINIMIZE pH SHIFT OF STORED MEDIA
8.1
Day 0 Day 1 Day 2 Day 3 Day 4 Day 7 Day 14
pH Deviation
8.0
7.9
7.8
7.7
7.6
7.5
7.4
7.3
Nalgene 500mL receiver
Brand M 500mL receiver
Brand C 500mL receiver
500mL glass bottle, 45mm neck
NALGENE RAPID-FLOW FILTERS MORE
800
Volume Filtered (mL), 100% CBS
1000mL filter units, 0.2µm PES
700
600
500
400
300
200
100
Spoke Design Filter M & C
Nalgene Rapid-Flow Filter
450
567
800
Nalgene Rapid-Flow Filter
Average Flow Rate (mL/min), DMEM+10%FBS
500mL filter units, 0.2µm PES
700
600
500
400
300
200
100
Spoke Design Filter C
NALGENE RAPID-FLOW IS FASTER
618
508
NALGENE RAPID-FLOW IS FASTER
800
Nalgene Rapid-Flow Filter
Average Flow Rate (mL/min), RPMI+10%CBS
1000mL filter units, 0.2µm PES
700
600
500
400
300
200
100
Spoke Design Filter M
698
471
Testing shows that Rapid-Flow filters deliver superior performance.
Faster filter.
Last line of defense.
• 0.1 micron filters guard against
mycoplasma contamination.
• 0.2 micron filters remove all bacteria.
• 0.45 and 0.8 micron filters for particle removal
and fluid clarification.
• 90 mm diameter filters provide large
surface area for serum and other
hard
-
to
-
filter solutions.
• Nalgene Certified. We guarantee it!
Easy to use shape.
• Ergonomic design.
• 1½ turn screw cap, tapered
sides and “grips” make the filter
units and storage bottles easy
to handle.
• Wide base improves stability on
the bench top.
Filter faster.
Unbeatable membranes.
No matter what aqueous fluid
you’re filtering, we have the right
membrane for you:
• PES (polyethersulfone)
• SFCA (surfactant-free
cellulose acetate)
• CN (cellulose nitrate)
• NYLON
Critical interface.
Multi-column design delivers
uniformity and consistency.
The Rapid-Flow membrane
support system, when
coupled with any of our
membrane options, offers
fast flow rates and higher
throughput.
References
: 1. Harrison KL, et al. Embryotoxicity of
micropore filters used in liquid sterilization. J In Vitro Fert
Embryo Transf.1990;7(6):347-350. 2. Wakeland JR, et
al. Toxicity to organ cultured hearts of media prepared
with disposable filter units. In Vitro. 1982;18(8):715-
718. 3. Engel F. The effect of membrane filters on
cultured cells. J Pharm Pharmacol. 1982;34:283-286.
Nalgene Storage Bottles
Perfect for storing filtered media and other
aqueous fluids. Leak-proof cap eliminates
pH shift for longer media life. Fits all Nalgene
Bottle Top Filters with 45mm neck thread.
Polyethersulfone (PES) is the ultimate tissue
culture membrane. It’s fast and clean. PES is
low protein binding, so there is less chance
of removing critical protein from your media.
It is hydrophilic, so no external wetting agents
or surfactants are needed, resulting in low
extractables. And it is fast, so you spend less
time waiting.
Nalgene Filters with PES Membrane
Capacity
ml
Pore
Size
μm
Membr.
Diam.
mm
Fits
Bottle
Neck
Size
Thermo
Scientific
Cat. No.
VWR Cat. No.
PES Filter Units
(12 units per pack)
50
0.2
50
Tube
564-0020
82030-938
150
0.1
50
—
565-0010
97014-830
150
0.2
50
—
565-0020
73520-982
150
0.45
50
—
165-0045
16211-053
250
0.1
50
—
568-0010
97014-832
250
0.2
50
—
568-0020
73520-988
250
0.45
50
—
168-0045
16211-061
500
0.1
75
—
566-0010
97014-834
500
0.2
75
—
566-0020
73520-984
500
0.45
75
—
166-0045
16211-054
500
0.2
90
—
569-0020
73520-990
500
0.45
90
—
169-0045
16211-064
1000
0.1
90
—
567-0010
89176-982
1000
0.2
90
—
567-0020
73520-986
1000
0.45
90
—
167-0045
16211-056
PES Bottle Top Filters
(12 units per pack)
150
0.2
50
33
596-3320
73520-996
150
0.45
50
33
296-3345
16211-073
150
0.2
50
45
596-4520
73520-998
150
0.45
50
45
296-4545
16211-077
500
0.2
75
33
595-3320
73520-992
500
0.45
75
33
295-3345
16211-066
500
0.2
75
45
595-4520
73520-994
500
0.45
75
45
295-4545
16211-068
1000
0.2
90
33
597-3320
73521-000
1000
0.2
90
45
597-4520
73521-002
Nalgene Storage Bottles
Capacity
ml
Number
Per Case
Thermo Scientific
Cat. No.
VWR Cat. No.
150
24
455-0150
28199-214
250
24
455-0250
28199-225
500
12
455-0500
28199-189
1000
12
455-1000
28199-246
(SFCA) contains none of the wetting agents
found in standard CA (cellulose acetate).
These wetting agents have been shown to
be toxic to specific cell lines in vitro (1-3).
SFCA also has lower protein binding than
does standard CA. If you must use CA for
media filtration, you owe it to your cell lines
to switch to SFCA. SFCA is available only in
Nalgene filter units and bottle top filters.
Nalgene Filters with SFCA Membrane
Capacity
ml
Pore
Size
μm
Membr.
Diam.
mm
Fits
Bottle
Neck
Size
Thermo
Scientific
Cat. No.
VWR Cat. No.
SFCA Filter Units
(12 units per pack)
150
0.2
50
—
155-0020
28199-622
150
0.45
50
—
155-0045
28199-633
250
0.2
50
—
157-0020
28199-677
250
0.45
50
—
157-0045
28199-688
500
0.2
75
—
156-4020
28199-585
500
0.45
75
—
156-4045
28199-600
500
0.2
90
—
162-0020
28198-505
500
0.45
90
—
162-0045
28198-507
1000
0.2
75
—
158-0020
28199-698
1000
0.45
75
—
158-0045
28199-712
1000
0.2
90
—
161-0020
28198-497
1000
0.45
90
—
161-0045
28198-498
SFCA Bottle Top Filters
(12 units per pack)
150
0.2
50
33
290-3320
28199-296
150
0.45
50
33
290-3345
28199-298
150
0.2
50
45
290-4520
28199-300
150
0.45
50
45
290-4545
28199-302
500
0.2
75
33
291-3320
28199-303
500
0.45
75
33
291-3345
28199-305
500
0.2
75
45
291-4520
28199-307
500
0.45
75
45
291-4545
28199-309
1000
0.2
90
33
292-3320
28199-312
1000
0.2
90
45
12
28199-317
Nylon is tough, alcohol resistant, and
has a low level of extractables. These
membrane factors make it the choice
for special applications.
Nalgene Filters
with Nylon Membrane
Capacity
ml
Pore
Size
μm
Membr.
Diam.
mm
Thermo
Scientific
Cat. No.
VWR Cat. No.
Nylon Filter Units
(12 units per pack)
150
0.2
50
150-0020
28199-338
150
0.45
50
150-0045
28199-349
250
0.2
50
153-0020
28199-371
250
0.45
50
153-0045
28199-382
500
0.2
75
151-4020
28199-359
500
0.45
75
151-4045
28199-360
500
0.2
90
163-0020
28198-510
1000
0.2
90
164-0020
28198-514
1000
0.2
75
154-0020
28199-417
1000
0.45
75
154-0045
28199-428
Cellulose nitrate (CN) membranes
are ideal for filtering and clarifying
buffers and other aqueous solutions
when protein binding is not a concern.
Nalgene CN membranes are TRITON
X
®
-free.
Nalgene Filters
with CN Membrane
Capacity
ml
Pore
Size
μm
Membr.
Diam.
mm
Thermo
Scientific
Cat. No.
VWR Cat. No.
CN Filter Units
(12 units per pack)
150
0.2
50
125-0020
28199-076
150
0.45
50
125-0045
28199-098
150
0.8
50
125-0080
28199-101
250
0.2
50
126-0020
28199-112
250
0.45
50
126-0045
28199-123
250
0.8
50
126-0080
28199-134
500
0.2
75
450-0020
28199-155
500
0.45
75
450-0045
28199-177
500
0.8
75
450-0080
28199-144
1000
0.2
75
127-0020
28199-268
1000
0.45
75
127-0045
28199-279
1000
0.8
75
127-0080
28199-280
Nalgene SFCA
Surfactant-free cellulose acetate
Nalgene PES
The best choice for cell culture
Nalgene NYLON
Alcohol resistant
Nalgene CN
For filtration of buffers
Ordering Information
© 2012 Thermo Fisher Scientific, Inc. All rights reserved.
Triton X is a registered trademark of Rohm and Haas.
All other trademarks are the property of Thermo Fisher
Scientific Inc. and its subsidiaries.
0512 5M Lit. No. 20192